Recombinant:Article Title: Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HeLa-M cells P. Pothukuchi et al, 2021 N/A HeLa-M hGH-FM-GFP cells De Gordon et al, 2010 N/A Reagent/resource Reference or source Identifier or catalog number A375MM cells A. Filograna et al, 2024, kind gift from Dr. D. Corda N/A Cos7 cells G. Grimaldi et al, 2022, kind gift from Dr. D. Corda N/A RPE cells F. Mascanzoni et al, 2024, kind gift from Dr. A. Colanzi N/A 6-month-old male Wistar rats Cardiological Centre in Moscow, Russia N/A Recombinant DNA ts045-VSVG-GFP Addgene Cat #11912 VSVG-GFP-RUSH Addgene Cat #65300 GPI-GFP-RUSH Addgene Cat #65296 VSVG-mCherry-RUSH Addgene Cat #65301 LAMP1-GFP-RUSH Kind gift from Dr. Franck Perez N/A E-cadherin-GFP-RUSH Kind gift from Dr. Franck Perez N/A Antibodies Monoclonal Mouse GM130 BD Biosciences Clone 35 Cat # 610822 RRID: AB_398141 Polyclonal Sheep antihuman TGN46 BioRad/AbD-Serotec Cat #AHP500G RRID:AB_323104 GOLGB1 (Giantin) Invitrogen PA5-52772 Anti-mouse, donkey Alexa Fluor 568 ThermoFisher Scientific Cat #A10037 RRID:AB_2534013 Anti-sheep, donkey Alexa Fluor 633 ThermoFisher Scientific Cat #A-21100 RRID:AB_2535754 Monoclonal Mouse GFP Abcam Cat #ab6556 RRID:AB_305564 Polyclonal Rabbit VSVG Bethyl Laboratories Inc. Cat #A190-131A; RRID:AB_155862 Protein A gold 10 nm Cell Microscopy Core, UMC Utrecht N/A Protein A gold 15 nm Cell Microscopy Core, UMC Utrecht N/A Oligonucleotides and other sequence-based reagents Chemicals, enzymes, and other reagents TransIT-LT1 Mirus Bio Cat # MIR2304 Lipofectamine LTX Life Technologies Cat # 15338100 Paraformaldehyde 8% Electron Microscopy Sciences Cat # 157-8 Glutaraldehyde 8% Electron Microscopy Sciences Cat # 16000 Biotin Sigma Aldrich Cat # B4501 Cycloheximide Sigma Aldrich Cat # 01810 D/D solubilizer Takara Bio Cat # 635054 Trichloroacetic acid Sigma Aldrich Cat # T6399 Mini-Protean Pecast Gels Biorad Cat # 4568081 ECL GE Healthcare Cat # RPN2209 © The Author(s) EMBO reports 13 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Complete mini EDTA free protease inhibitor cocktail Roche Cat # 4693159001 Software Zen software system Carl Zeiss Metamorph 7.7.3.0 Universal Imaging ImageJ Open source Prism 8.0 GraphPad Other
Microscopy:Article Title: Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HeLa-M cells P. Pothukuchi et al, 2021 N/A HeLa-M hGH-FM-GFP cells De Gordon et al, 2010 N/A Reagent/resource Reference or source Identifier or catalog number A375MM cells A. Filograna et al, 2024, kind gift from Dr. D. Corda N/A Cos7 cells G. Grimaldi et al, 2022, kind gift from Dr. D. Corda N/A RPE cells F. Mascanzoni et al, 2024, kind gift from Dr. A. Colanzi N/A 6-month-old male Wistar rats Cardiological Centre in Moscow, Russia N/A Recombinant DNA ts045-VSVG-GFP Addgene Cat #11912 VSVG-GFP-RUSH Addgene Cat #65300 GPI-GFP-RUSH Addgene Cat #65296 VSVG-mCherry-RUSH Addgene Cat #65301 LAMP1-GFP-RUSH Kind gift from Dr. Franck Perez N/A E-cadherin-GFP-RUSH Kind gift from Dr. Franck Perez N/A Antibodies Monoclonal Mouse GM130 BD Biosciences Clone 35 Cat # 610822 RRID: AB_398141 Polyclonal Sheep antihuman TGN46 BioRad/AbD-Serotec Cat #AHP500G RRID:AB_323104 GOLGB1 (Giantin) Invitrogen PA5-52772 Anti-mouse, donkey Alexa Fluor 568 ThermoFisher Scientific Cat #A10037 RRID:AB_2534013 Anti-sheep, donkey Alexa Fluor 633 ThermoFisher Scientific Cat #A-21100 RRID:AB_2535754 Monoclonal Mouse GFP Abcam Cat #ab6556 RRID:AB_305564 Polyclonal Rabbit VSVG Bethyl Laboratories Inc. Cat #A190-131A; RRID:AB_155862 Protein A gold 10 nm Cell Microscopy Core, UMC Utrecht N/A Protein A gold 15 nm Cell Microscopy Core, UMC Utrecht N/A Oligonucleotides and other sequence-based reagents Chemicals, enzymes, and other reagents TransIT-LT1 Mirus Bio Cat # MIR2304 Lipofectamine LTX Life Technologies Cat # 15338100 Paraformaldehyde 8% Electron Microscopy Sciences Cat # 157-8 Glutaraldehyde 8% Electron Microscopy Sciences Cat # 16000 Biotin Sigma Aldrich Cat # B4501 Cycloheximide Sigma Aldrich Cat # 01810 D/D solubilizer Takara Bio Cat # 635054 Trichloroacetic acid Sigma Aldrich Cat # T6399 Mini-Protean Pecast Gels Biorad Cat # 4568081 ECL GE Healthcare Cat # RPN2209 © The Author(s) EMBO reports 13 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Complete mini EDTA free protease inhibitor cocktail Roche Cat # 4693159001 Software Zen software system Carl Zeiss Metamorph 7.7.3.0 Universal Imaging ImageJ Open source Prism 8.0 GraphPad Other
Sequencing:Article Title: Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HeLa-M cells P. Pothukuchi et al, 2021 N/A HeLa-M hGH-FM-GFP cells De Gordon et al, 2010 N/A Reagent/resource Reference or source Identifier or catalog number A375MM cells A. Filograna et al, 2024, kind gift from Dr. D. Corda N/A Cos7 cells G. Grimaldi et al, 2022, kind gift from Dr. D. Corda N/A RPE cells F. Mascanzoni et al, 2024, kind gift from Dr. A. Colanzi N/A 6-month-old male Wistar rats Cardiological Centre in Moscow, Russia N/A Recombinant DNA ts045-VSVG-GFP Addgene Cat #11912 VSVG-GFP-RUSH Addgene Cat #65300 GPI-GFP-RUSH Addgene Cat #65296 VSVG-mCherry-RUSH Addgene Cat #65301 LAMP1-GFP-RUSH Kind gift from Dr. Franck Perez N/A E-cadherin-GFP-RUSH Kind gift from Dr. Franck Perez N/A Antibodies Monoclonal Mouse GM130 BD Biosciences Clone 35 Cat # 610822 RRID: AB_398141 Polyclonal Sheep antihuman TGN46 BioRad/AbD-Serotec Cat #AHP500G RRID:AB_323104 GOLGB1 (Giantin) Invitrogen PA5-52772 Anti-mouse, donkey Alexa Fluor 568 ThermoFisher Scientific Cat #A10037 RRID:AB_2534013 Anti-sheep, donkey Alexa Fluor 633 ThermoFisher Scientific Cat #A-21100 RRID:AB_2535754 Monoclonal Mouse GFP Abcam Cat #ab6556 RRID:AB_305564 Polyclonal Rabbit VSVG Bethyl Laboratories Inc. Cat #A190-131A; RRID:AB_155862 Protein A gold 10 nm Cell Microscopy Core, UMC Utrecht N/A Protein A gold 15 nm Cell Microscopy Core, UMC Utrecht N/A Oligonucleotides and other sequence-based reagents Chemicals, enzymes, and other reagents TransIT-LT1 Mirus Bio Cat # MIR2304 Lipofectamine LTX Life Technologies Cat # 15338100 Paraformaldehyde 8% Electron Microscopy Sciences Cat # 157-8 Glutaraldehyde 8% Electron Microscopy Sciences Cat # 16000 Biotin Sigma Aldrich Cat # B4501 Cycloheximide Sigma Aldrich Cat # 01810 D/D solubilizer Takara Bio Cat # 635054 Trichloroacetic acid Sigma Aldrich Cat # T6399 Mini-Protean Pecast Gels Biorad Cat # 4568081 ECL GE Healthcare Cat # RPN2209 © The Author(s) EMBO reports 13 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Complete mini EDTA free protease inhibitor cocktail Roche Cat # 4693159001 Software Zen software system Carl Zeiss Metamorph 7.7.3.0 Universal Imaging ImageJ Open source Prism 8.0 GraphPad Other
Electron Microscopy:Article Title: Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HeLa-M cells P. Pothukuchi et al, 2021 N/A HeLa-M hGH-FM-GFP cells De Gordon et al, 2010 N/A Reagent/resource Reference or source Identifier or catalog number A375MM cells A. Filograna et al, 2024, kind gift from Dr. D. Corda N/A Cos7 cells G. Grimaldi et al, 2022, kind gift from Dr. D. Corda N/A RPE cells F. Mascanzoni et al, 2024, kind gift from Dr. A. Colanzi N/A 6-month-old male Wistar rats Cardiological Centre in Moscow, Russia N/A Recombinant DNA ts045-VSVG-GFP Addgene Cat #11912 VSVG-GFP-RUSH Addgene Cat #65300 GPI-GFP-RUSH Addgene Cat #65296 VSVG-mCherry-RUSH Addgene Cat #65301 LAMP1-GFP-RUSH Kind gift from Dr. Franck Perez N/A E-cadherin-GFP-RUSH Kind gift from Dr. Franck Perez N/A Antibodies Monoclonal Mouse GM130 BD Biosciences Clone 35 Cat # 610822 RRID: AB_398141 Polyclonal Sheep antihuman TGN46 BioRad/AbD-Serotec Cat #AHP500G RRID:AB_323104 GOLGB1 (Giantin) Invitrogen PA5-52772 Anti-mouse, donkey Alexa Fluor 568 ThermoFisher Scientific Cat #A10037 RRID:AB_2534013 Anti-sheep, donkey Alexa Fluor 633 ThermoFisher Scientific Cat #A-21100 RRID:AB_2535754 Monoclonal Mouse GFP Abcam Cat #ab6556 RRID:AB_305564 Polyclonal Rabbit VSVG Bethyl Laboratories Inc. Cat #A190-131A; RRID:AB_155862 Protein A gold 10 nm Cell Microscopy Core, UMC Utrecht N/A Protein A gold 15 nm Cell Microscopy Core, UMC Utrecht N/A Oligonucleotides and other sequence-based reagents Chemicals, enzymes, and other reagents TransIT-LT1 Mirus Bio Cat # MIR2304 Lipofectamine LTX Life Technologies Cat # 15338100 Paraformaldehyde 8% Electron Microscopy Sciences Cat # 157-8 Glutaraldehyde 8% Electron Microscopy Sciences Cat # 16000 Biotin Sigma Aldrich Cat # B4501 Cycloheximide Sigma Aldrich Cat # 01810 D/D solubilizer Takara Bio Cat # 635054 Trichloroacetic acid Sigma Aldrich Cat # T6399 Mini-Protean Pecast Gels Biorad Cat # 4568081 ECL GE Healthcare Cat # RPN2209 © The Author(s) EMBO reports 13 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Complete mini EDTA free protease inhibitor cocktail Roche Cat # 4693159001 Software Zen software system Carl Zeiss Metamorph 7.7.3.0 Universal Imaging ImageJ Open source Prism 8.0 GraphPad Other
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